Dott. Matteo Cuccato
- SCIENZE VETERINARIE PER LA SALUTE ANIMALE E LA SICUREZZA ALIMENTARE
- Phd: 35th cycle
- Department of Veterinary Sciences
- Matriculation number: 911236
- ORCID: orcid.org/0000-0001-8431-0695
Phd thesis
PhD thesis title: "Application of Next-Generation Sequencing (NGS) as a new approach to study antimicrobial effects in farm animals".
Summary
Antimicrobials (AMs) are widely use to prevent and treat infectious diseases. The new European regulatory scenario asks for a substantial reduction of AMs use in order to control antimicrobial resistance (AMR) spreading. Therefore, this dissertation aimed to investigate poultry and dairy cows farming systems applying a different Next-generation sequencing (NGS) approach. In poultry farming, ensuring gut health and efficient growth performance has become one of the biggest challenges. Investigating the AMs effects on gut microbiota and host intestinal barrier allows the identification of the mechanisms underlying the intricate relationship between host, microbiota and AMs. The introduction of new limitations in AMs use requires the implementation and the improvement of the diagnostic tools, available for AMs identification in food matrixes. In this context, this thesis firstly investigated the role of AMs at gut barrier level using 16S metabarcoding, associated with histopathology, IHC and gene expression techniques. The results of this research suggest an influence of AMs on the gut microbiota, characterised by dysbiosis. Moreover, the identification of intestinal microscopic lesions and their association with microbiota alterations revealed a negative effect of AMs on the gut barrier of treated broilers. On the other hand, in dairy cows farming there is an urgent need to support new studies with the aim of identifying new biomarkers for an early diagnosis of mastitis. An early identification of subclinical forms will allow the application of preventive pharmacological treatments and efficient biosecurity measures. In this study, the role of miRNAs vehiculated by EVs has been investigated in the modulation of mastitis and in their application as potential early predictors of subclinical mastitis. A panel of 4 main miRNAs has been proposed. According to the functional analysis, their function is mainly related to immune and inflammatory functions. In conclusion, it is possible to confirm that NGS technology has been a useful choice in the investigations of the relationship between host, microbiota and drugs. NGS application can be a powerful tool to find new solutions and support the new requirements derived from the veterinary scenario related to AMR and AMs use.
Research activities
Pubblicazioni su rivista
Cuccato M, Divari S, Sacchi P, Girolami F, Cannizzo FT.
“MALDI-TOF mass spectrometry profiling of bovine skim milk for subclinical mastitis detection.”
Frontiers in Veterinary Science, 2022. 9:1009928.
doi: 10.3389/fvets.2022.1009928
Laconi A, Tolosi R, Mughini-Gras L, Cuccato M, Cannizzo FT, Piccirillo A.
“Amoxicillin and thiamphenicol treatments may influence the co‐selection of resistance genes in the chicken gut microbiota.”
Scientific Reports, 2022. 12:20413.
doi: 10.1038/s41598-022-24927-7
Divari S, Cuccato M, Fanelli A, Cannizzo FT.
“Development of a droplet digital PCR assay to detect illicit glucocorticoid administration in bovine.”
Plos One, 2022. 17(7): e0271613.
doi: 10.1371/journal.pone.0271613
Cuccato M, Scaglione FE, Centelleghe C, Divari S, Biolatti B, Pregel P, Cannizzo FT.
“Assessment of Antimicrobial Effects on Broiler Gut Barrier Through Histopathology and Immunohistochemistry of Tight-Junction Proteins.”
Frontiers in Veterinary Science, 2022. 9:830073.
doi: 10.3389/fvets.2022.830073
Cuccato M, Rubiola S, Giannuzzi D, Grego E, Pregel P, Divari S, Cannizzo FT.
“16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents”
Antibiotics, 2021; 10(2): 146.
doi: 10.3390/antibiotics10020146
Abstract in atti di convegno
Laconi A, Tolosi R, Cuccato M, Mughini-Gras L, Cannizzo FT, Tilli G, Piccirillo A.
“Amoxicillin and thiamphenicol treatments enhance the abundance of antimicrobial resistance genes in the chicken gut.”
Poster, 11th International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM), 11-14 Settembre 2022. Madrid (Spain).
Cannizzo FT, Divari S, Cuccato M, Ciaramita S, Meliota F, Campelli D, Biolatti B, Biolatti PG, Bollo E.
“Actinobacillus pleuropneumoniae in swine: lung lesions scoring and multiplex PCR identification in Piedmont farms.”
Poster, Annual ESVP (European Society of Veterinary Pathology) Congress, 7-10 Settembre 2022, Athens (Greece).
Laconi A, Tolosi R, Mughini-Gras L, Cuccato M, Carraro L, Cannizzo FT, Tilli G, Piccirillo A.
"Chicken Gut Microbiota Dynamics after Amoxicillin and Thiamphenicol Treatment."
Poster, American Association Avian Pathologist (AAAP) Annual Meeting, 29 Luglio - 2 Agosto 2022 Philadelphia (USA).
Cuccato M, Pawlowski K, Faulconnier Y, Pires J, Bes S, Sacchi P, Cannizzo FT, Leroux C.
“Milk fat globule miRNome response to an LPS challenge in Holstein cows.”
Comunicazione orale, 75° Convegno SISvet (Società Italiana delle Scienze Veterinarie) 15-18 Giugno 2022, Lodi (IT).
Cannizzo FT, Divari S, Cuccato M, Ciaramita S, Meliota F, Campelli D, Biolatti B, Biolatti PG, Bollo E.
“Swine plueropneumonia: lung scoring and Actinobacillus pleuropneumoniae PCR identification in Piedmont farms.”
Comunicazione orale, 75° Convegno SISvet (Società Italiana delle Scienze Veterinarie) 15-18 Giugno 2022, Lodi (IT).
Prampolini A, Cuccato M, Magri M, Cannizzo FT, Addis MF, Bullone M.
“Fecal microbiome and mucosal inflammation in horses with recurrent colic due to large colon inflammatory pathologies.”
Comunicazione orale, 75° Convegno SISvet (Società Italiana delle Scienze Veterinarie) 15-18 Giugno 2022, Lodi (IT).
Rubiola S, Cuccato M, Divari S, Cannizzo FT, Bullone M.
“The equine nasal resistome: a next generation sequencing approach.”
Comunicazione orale, 75° Convegno SISvet (Società Italiana delle Scienze Veterinarie) 15-18 Giugno 2022, Lodi (IT).
Cuccato M, Scaglione FE, Pregel P, Laconi A, Perona G, Nurisso S, Sereno A, Divari S, Piccirillo A, Cannizzo FT.
“Effetti delle profilassi antimicrobiche sul microbiota intestinale e sulla barriera intestinale in polli broiler.”
Comunicazione orale, VI Simposio Scientifico SIPA (Società Italiana di Patologia Aviare) 19 Novembre 2021, Bologna (IT).
Laconi A, Tolosi R, Cuccato M, Mughini-Gras L, Cannizzo FT, Tilli G, Piccirillo A.
“Antimicrobial Resistance Dynamics in the Chicken Gut after Amoxicillin and Thiamphenicol Treatments”
Poster, IMED (International Meeting on Emerging Diseases and Surveillance) congress 2021, 4-6 Novembre 2021, virtual edition.
International Journal of Infectious Diseases 116 (2022) S1–S130 doi: 10.1016/j.ijid.2021.12.015
Cuccato M, Divari S, Scaglione FE, Centelleghe C, Sereno A, Pregel P, Cannizzo FT.
“Effects of different antimicrobials prophylaxis protocols on intestinal histology and microbiota composition in broiler chickens.”
Comunicazione orale, 74° Convegno SISvet (Società Italiana delle Scienze Veterinarie) 23-26 Giugno 2021, virtual edition.
Apostolakos I, Fasolato L, Cuccato M, Ferraresso J, Rizzo R, Zago M, Piccirillo A.
“Trends of resistance to extended-spectrum β-lactams in commensal and clinical Escherichia coli from the broiler production pyramid.”
Comunicazione orale, 9th International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM 2018), Rome, Italy, 16-18 Ottobre 2018, Roma (IT).
Apostolakos I, Fasolato L, Cuccato M, Ferraresso J, Rizzo R, Zago M, Piccirillo A.
“Occurrence of β-lactam resistant Escherichia coli along the broiler production chain.” Comunicazione orale, III Simposio Scientifico SIPA (Società Italiana di Patologia Aviare) 14 Settembre 2018, Parma (IT).